Structural Insights into a Unique Dimeric DEAD-Box Helicase CshA that Promotes RNA Decay

Authors: Jennifer Huen, Chia-Liang Lin,Bagher Golzarroshan, Wan-Li Yi,Wei-Zen Yang, Hanna S. Yuan

In Brief: Huen et al. report the crystal structure of CshA, a unique dimeric DEAD-box helicase. CshA binds RNA with or without ATP/ADP, as RNA remain bound with CshA during ATP hydrolysis cycles. RNA is thus degraded processively through cooperation between exoribonucleases and CshA in the RNA-degrading machinery.

Highlights CshA is a unique dimeric DEAD-box helicase in the RNA degradosome for RNA decay The crystal and SAXS structures of CshA reveal a flexible V-shaped structure Dimerization of CshA produces a helicase that binds RNA with or without ATP Dimeric CshA cooperates with exoribonucleases to unwind and degrade RNA Accession Numbers 5IVL Huen et al., 2017, Structure 25, 1–13 March 7, 2017 ª 2017 Elsevier Ltd. http://dx.doi.org/10.1016/j.str.2017.01.012

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