Crystallization and preliminary X-ray diffraction analysis of malic enzyme from pigeon liver
Li-Chu Tsai, Chen-Chin Kuo, Wei-Yuan Chou, Gu-Gang Chang and Hanna S. Yuan
SUMMARY
Recombinant pigeon-liver malic enzyme was expressed in Escherichia coli and purified to homogeneity. Two different crystal forms were grown by the hanging-drop vapour-diffusion method. Both types of crystals belong to the tetragonal space group P42 22, with unit-cell dimensions a = b = 163.8, c = 174.3 Å for the octahedral crystals and a = b = 124.5, c = 179.2 Å for the rod-like crystals. X-ray diffraction data were collected at 100 K using a synchrotron-radiation X-ray source. The Matthews parameter suggests that there are four and two molecules per asymmetric unit for the larger and the smaller tetragonal unit cells, respectively.Acta Cryst. (1999). D55, 1930-1932
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