Publications (1994 to present)

　

2006

　

Doudeva, L. G., Huang, H., Hsia, K.-C., Shi, Z., Li, C.-L, Shen, Y. and Yuan*, H. S. (2006) Crystal structural analysis and metal-dependent stability and activity studies of the ColE7 endonuclease domain in complex with DNA/Zn²⁺ or inhibitor/Ni²⁺. Protein Sci. 15, 269-280.

　

Cheng, Y.-S., Shi, Z., Doudeva, L. G., Yang, W.-Z., Chak, K.-F. and Yuan*, H. S. (2006) High-resolution crystal structure of a truncated ColE7 translocation domain: Implications for colicin transport across membranes. J. Mol. Biol. 356, 22-31.

　

2005

　

Hsuan Liu, Hsien-Wei Peng, Yi-Sheng Cheng, Hanna S. Yuan and Hsin-Fang Yang-Yen. (2005)  Stabilization and Enhancement of the Antiapoptotic Activity of Mcl-1 by TCTP. Mol. Cell. Biol. Apr, 3117–3126.

 

Shi, Z –H., Chak. K –F, and Yuan*, H, S.(2005) Identification of an Essential Cleavage Site in ColE7 Required for Import and Killing of Cells. J. Biol. Chem. 280, 24663-24668.

   

Hsia, K.-C., Li, C.-L. and Yuan*, H. S. (2005) Structural and functional insight into the sugar-nonspecific nucleases in host defense. Curr. Opin. Struct. Biol,. 15, 126-134.

　　

Lin, Y.-H., Liao, C.-C., Liang, P.-H., Yuan, H. S. and Chak, K.-F (2004). Involvement of colicin in the limited protection of the colicin producing cells against bacteriophage. Biochem. Biophys. Res. Commun. 318, 81-87.

　

2004

　

Hsia, K.-C., Chak, K.-F., Liang, P.-H., Cheng, Y.-S., Ku, W.-Y. and Yuan*, H. S.(2004) DNA binding and degradation by the H-N-H protein ColE7. Structure 12, 205-214

 

2003

 

Tsai, L.-C., Shyur, L.-F., Lee, S.-H. Lin, S.-S. and Yuan*, H. S.(2003) Crystal structure of a natural circularly-permutated jellyroll protein: 1,3-1,4-beta-D-glucanase from Fibrobacter succinogenes. J. Mol. Biol. 330, 607-620

 

Li, C.-L., Hor, L.-I., Chang, Z.-F., Tsai, L.-C., Yang, W.-Z. and Yuan*, H. S.(2003) DNA binding and cleavage by the periplasmic nuclease Vvn- A novel structure with a known active site. EMBO J. 22, 4014-4025

 

2002

　

Chang, S.-J., Hsieh, S.-Y., Yuan, H. S. and Chak, K.-F. (2002) Characterization of the specific cleavage of ceiE7-mRNA of the bactericidal ColE7 operon. Biochem. Biophys. Res. Commun. 299, 613-620

　

Cheng, Y.-S., Hsia, K.-C., Doudeva, L. G., Kin-Fu Chak and Yuan, H. S. (2002) The crystal structure of the nuclease domain of colicin E7 suggests a mechanism for binding to double-stranded DNA by the H-N-H endonucleases. J. Mol. Biol. 324, 227-236.

　

Sui, M. J., Tsai, L. C., Hsia, K.-C., Doudeva, L. G., Ku, W.-Y., Han, G. W. and Yuan, H. S. (2002) Metal ions and phosphate binding in the H-N-H motif: Crystal structures of the nuclease domain of ColE7/Im7 in complex with a phosphate ion and different divalent metal ions. Protein Sci. 11, 2947-2957

　

Cheng, H.-L., Tsai, L.-C., Lin, S.-S., Yuan, H. S., Yang, N.-S., Lee, S.-H. and Shyur, L.-F. (2002) Mutagenesis of Trp54 and Trp203 residues on Fibrobacter Succinogens 1,3-1,4-b-D-glucanase significantly affects catalytic activities of the enzyme. Biochemistry 41, 8759-8766

　

Ku, W.-Y., Liu, Y.-W., Hsu, Y.-C., Liao, C.-C., Liang, P.-H., Yuan, H.-S. and Chak, K.-F.(2002) The zinc ion in the HNH motif of the endonuclease domain of colicin E7 is not required for DNA binding but is essential for DNA hydrolysis. Nucleic Acid Res. 30, 1670-1678

　

Yang Z., Zhang H., Hung H. C., Kuo, C. C., Tsai, L. C., Yuan, H. S., Chou, W. Y., Chang, G. G., Tong, L. (2002) Structural studies of the pigeon cytosolic NADP(+)-dependent malic enzyme. Protein Sci., 11:332-41

2001

　

Chen, J. L., Tsai, L. C., Wen, T. N., Tang, J. B., Yuan, H. S. and Shyur, L. F. (2001) Directed mutagenesis of specific active site residues on Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase significantly affects catalysis and enzyme structural stability. J. Biol. Chem., 276 , 17895-17901

　

Tsai, L. C., Shyur, L. F., Lin, S. S., Yuan, H. S. (2001) Crystallization and preliminary X-ray diffraction analysis of the 1,3-1,4-beta-D-glucanase from Fibrobacter succinogenes. Acta Cryst. D57: 1303-1306

　

2000

　

　

　

　

　

　

Cheng, Y.-S., Yang, W.-Z., Johnson, R. C. and Yuan, H. S. (2001) Structure analysis of the transcriptional activation region on Fis: Crystal structures of Six Fis Mutants with Different activation properties.  J. Mol. Biol., 302, 1139-1151 

1999

Ko, T.-P., Liao, C.-C., Ku, W.-Y., Chak, K.-F. and Yuan, H. S. (1999) The crystal structure of the DNase domain of colicin E7 in complex with its inhibitor Im7 protein. Structure, 7, 91-102.

　

Tsai, L.-C, Kuo, C.-C., Chou, W.-Y., Chang, G.-G. and Yuan, H. S. (1999) Crystallization and preliminary x-ray diffraction analysis of malic enzyme from pigeon liver. Acta Cryst. D 55, 1930-1932.

　

Ko, T. P., Yang, W.-Z, Wu, S.-P., Hsin, T., and Yuan, H. S. (1999) The preliminary crystallographic analysis of the open-form E. coli tyrosine aminotransfer ase bound with a cofactor pyridoxal 5'-phosphate. Acta Cryst. D55, 1474-1477.

　

Lu, F.-M., Yuan, H. S., Hsu, Y.-C., Chang, S.-J., Chak, K.-F. (1999) Hierarchical order of critical residues on the immunity-determining region of the Im7 protein which confer specific immunity to its cognate colicin. Biochem-Biophys-Res-Commun. 264, 69-75.

　

Chang, S.-L., Chao, C.-H., Huang, Y.-S., Jean, Y.-C., Sheu, H.-S., Liang, F.-J., Chen, C.-K., Yuan, H. S. (1999) Inolving the x-ray phase problem of macromolecular crystals by stereoscopic multi-beam imaging. Acta. Cryst. A55, 933-938.

　

Hsu, W.-H., Chien, F.-T., Hsu, C.-L., Wang, T.-C., Yuan, H. S., and Wang, W.-C. (1999) Expression, crystallization and preliminary x-ray diffraction studies of N- carbamyl-D-amino-acid amidohydrolase from Agrobacterium radiobacter. Acta. Cryst. D55, 694-695.

1998

Yang, W.-Z, Ko, T. P., Corselli, L., Johnson, R. C. and Yuan, H. S. (1998) The conversion from a beta-strand to an alph-helix induced by a single-site mutation identified in the crystal structures of Fis mutant Pro26Ala. Protein Sci 7, 1875-1883.

1997

Safo, M. K., Yang, W.-Z., Corselli, L., Crampton, S., Yuan, H. S., and Johnson, R. C. (1997)  The transactivation region of the Fis protein that controls site-specific DNA inversion contains extended mobile beta-hairpin arms. EMBO J. 16, 6860-6873.

Hsieh, S.-H., Ko, T.-P., Tseng, M.-Y., Ku, W.-Y., Chak, K.-F, and Yuan, H. S. (1997) A novel role of ImmE7 in the autoregulatory expression of ColE7 operon and identification of possible ribonuclease active sites in the crystal structure of dimeric ImmE7. EMBO J. 16, 1444-1454.

Ko,T.-P. and Yuan, H. S . (1997) A brief introduction to protein crystallography using ImmE7 as an example. Chemistry 55, 1-8.

1996

Chak, K.-F., Safo, M. K., Ku, W.-Y., Hsieh, S.-Y., and Yuan, H. S. (1996)The crystal structure of the ImmE7 protein suggests a possible colicin-interacting surface. Proc. Natl. Acad. Sci. USA, 93, 6437-6442.

Ko, T.-P., Ku, W.-Y., and Yuan, H. S. (1996) The crystal structure of dimeric ImmE7 presents possible ribonuclease active sites. Proceedings of the fourth R.O.C.-Japan Joint Seminar on crystallography ,43-54 (1996).

1995

Ku, W. Y., Wang, C. S., Chen, J. Y., Chia, K. F., Safo, M. K., and Yuan, H. S. (1995) Crystallization and preliminary crystallographic analysis of ImmE7 protein of Colicin E7. Proteins, Struc. Func. & Genet. 23, 588-590.

1994

Yuan, H. S., Yang, W.-Z., Wang, S. S., Finkel, S. E. and Johnson, R. C. (1994) The structure of Fis mutant Pro61-Ala illustrates that the kink within the long a-helix is not due to the presence of the proline residue. J. Biol. Chem. 269, 46, 28947-28954,.

Yuan, H. S., Stevens, R. C., Kotzle, T. F. and Bau, R(1994) Determination of the absolute configuration of (S)-(+)-neopentyl-1-d alcohol by neutron diffraction study of strychnium neopentylphthalate. Proc. Natl. Acad. Sci. USA , 91, 12872-12876,.