HNH endonuclease bacterial toxin Colicin E7

Factor for  inversion stimulation Fis

Periplasmaic endonuclease Vvn

Other Enzymes

Tudor-SN

Pnpase

 

Colicin

 1CEI

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STRUCTURE DETERMINATION OF THE COLICIN E7 IMMUNITY PROTEIN (IMME7) THAT BINDS SPECIFICALLY TO THE DNASE-TYPE COLICIN E7 AND INHIBITS ITS BACTERIOCIDAL ACTIVITY

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-->  Characteristics  

Release Date:  12-Mar-1997  Exp. Method:  X Ray Diffraction 

Resolution:  1.80 Å

  Classification  

Antibacterial Protein    

  Compound  

Polymer: 1   Molecule: COLICIN E7 IMMUNITY PROTEIN   Chains: A  

  Authors  

Chak, K.-F.,  Safo, M.K.,  Ku, W.-Y.,  Hsieh, S.-Y.,  Yuan, H.S.*

 

 1UNK

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STRUCTURE OF COLICIN E7 IMMUNITY PROTEIN

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Characteristics  

Release Date:  07-Jan-1998  Exp. Method:  X Ray Diffraction 

Resolution:  1.80 Å

  Classification  

Immunity Protein    

  Compound  

Polymer: 1   Molecule: COLICIN E7   Chains: A,B,C,D  

  Authors  

Ko, T.-P.,  Hsieh, S.-Y.,  Ku, W.-Y.,  Tseng, M.-Y.,  Chak, K.-F.,  Yuan, H.S.*

 

 7CEI

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THE ENDONUCLEASE DOMAIN OF COLICIN E7 IN COMPLEX WITH ITS INHIBITOR IM7 PROTEIN

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Characteristics  

Release Date:  17-Sep-1999  Exp. Method:  X Ray Diffraction 

Resolution:  2.30 Å

  Classification  

Immune System    

  Compound  

Polymer: 1   Molecule: PROTEIN (COLICIN E7 IMMUNITY PROTEIN)   Chains: A  
Polymer:
 2   Molecule: PROTEIN (COLICIN E7 IMMUNITY PROTEIN)   Fragment: ENDONUCLEASE DOMAIN   Chains: B  

  Authors  

Ko, T.P.,  Liao, C.C.,  Ku, W.Y.,  Chak, K.F.,  Yuan, H.S.*

 

 1M08

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Crystal structure of the unbound nuclease domain of ColE7

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Characteristics  

Release Date:  11-Dec-2002  Exp. Method:  X Ray Diffraction 

Resolution:  2.10 Å

  Classification  

Hydrolase    

  Compound  

Polymer: 1   Molecule: Colicin E7   Fragment: Nuclease Domain   Chains: A,B   EC no.: 3.1.-.-   

  Authors  

Cheng, Y.S.,  Hsia, K.C.,  Doudeva, L.G.,  Chak, K.F.,  Yuan, H.S.*

 

 1MZ8

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CRYSTAL STRUCTURES OF THE NUCLEASE DOMAIN OF COLE7/IM7 IN COMPLEX WITH A PHOSPHATE ION AND A ZINC ION

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Characteristics  

Release Date:  23-Dec-2002  Exp. Method:  X Ray Diffraction 

Resolution:  2.00 Å

  Classification  

Toxin     Hydrolase/protein Binding    

  Compound  

Polymer: 1   Molecule: Colicin E7 immunity protein   Chains: A,C  
Polymer:
 2   Molecule: Colicin E7   Fragment: nuclease domain   Chains: B,D   EC no.: 3.1.-.-   

  Authors  

Sui, M.J.,  Tsai, L.C.,  Hsia, K.C.,  Doudeva, L.G.,  Ku, W.Y.,  Han, G.W.,  Yuan, H.S.*

   

 1PT3

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Crystal structures of nuclease-ColE7 complexed with octamer DNA

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Characteristics  

Release Date:  30-Mar-2004  Exp. Method:  X Ray Diffraction 

Resolution:  2.50 Å

  Classification  

Hydrolase/DNA   

  Compound  

Polymer: 1   Molecule: 5'-GCGATCGC-3'   Chains: C,D,E,F,G,H  
Polymer:
 2   Molecule: Colicin E7   Fragment: residues 449-576   Chains: A,B   EC no.: 3.1.-.-   

  Authors  

Hsia, K.C.,  Chak, K.F.,  Cheng, Y.S.,  Ku, W.Y.,  Yuan, H.S.*

 

 1ZNS

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Crystal structure of N-ColE7/12-bp DNA/Zn complex

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Characteristics  

Release Date:  14-Mar-2006  Exp. Method:  X Ray Diffraction 

Resolution:  2.50 Å

  Classification  

Hydrolase/DNA    

  Compound  

Polymer: 1   Molecule: 5'-D(*CP*GP*GP*GP*AP*TP*AP*TP*CP*CP*CP*G)-3'   Chains: B,C  
Polymer:
 2   Molecule: Colicin E7   Fragment: nuclease domain   Mutation: H545E   Chains: A   EC no.: 3.1.-.-   

  Authors  

Doudeva, L.G.,  Huang, H.,  Hsia, K.C.,  Shi, Z.,  Li, C.L.,  Shen, Y.,  Yuan, H.S.*

 

 1ZNV

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How a His-metal finger endonuclease ColE7 binds and cleaves DNA with a transition metal ion cofactor

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Characteristics  

Release Date:  14-Mar-2006  Exp. Method:  X Ray Diffraction 

Resolution:  2.00 Å

  Classification  

Hydrolase/protein Binding    

  Compound  

Polymer: 1   Molecule: Colicin E7 immunity protein   Chains: A,C  
Polymer:
 2   Molecule: Colicin E7   Fragment: Nuclease domain   Mutation: H545E   Chains: B,D   EC no.: 3.1.-.-   

  Authors  

Doudeva, L.G.,  Huang, H.,  Hsia, K.C.,  Shi, Z.,  Li, C.L.,  Shen, Y.,  Yuan, H.S.

 

 2AXC

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Crystal structure of ColE7 translocation domain

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 Characteristics  

Release Date:  14-Mar-2006  Exp. Method:  X Ray Diffraction 

Resolution:  1.70 Å

  Classification  

Hydrolase    

  Compound  

Polymer: 1   Molecule: Colicin E7   Fragment: N-terminal Translocation domain   Chains: A   EC no.: 3.1.-.-   

  Authors  

Cheng, Y.S.,  Shi, Z.,  Doudeva, L.G.,  Yang, W.Z.,  Chak, K.F.,  Yuan, H.S.

 

 2IVH

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CRYSTAL STRUCTURE OF THE NUCLEASE DOMAIN OF COLE7 (H545Q MUTANT) IN COMPLEX WITH AN 18-BP DUPLEX DNA

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Characteristics  

Release Date:  02-Jan-2007  Exp. Method:  X Ray Diffraction 

Resolution:  2.80 Å

  Classification  

Hydrolase/DNA

  Compound  

Polymer: 1   Molecule: 5'-D(*GP*GP*AP*AP*TP*TP*CP*GP*AP*TP *CP*GP*AP*AP*TP*TP*CP*C)-3'   Chains: B,C  
Polymer:
 2   Molecule: COLCIN-E7   Fragment: NUCLEASE DOMAIN, RESIDUES 449-576   Mutation: YES   Chains: A   EC no.: 3.1.-.-   

  Authors  

Wang, Y.T.,  Yang, W.J.,  Li, C.L.,  Doudeva, L.G.,  Yuan, H.S.

 

 2JAZ

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CRYSTAL STRUCTURE OF THE MUTANT N560D OF THE NUCLEASE DOMAIN OF COLE7 IN COMPLEX WITH IM7

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Characteristics  

Release Date:  03-Apr-2007  Exp. Method:  X Ray Diffraction 

Resolution:  2.03 Å

  Classification  

Hydrolase/inhibitor Complex    

  Compound  

Polymer: 1   Molecule: COLICIN E7 IMMUNITY PROTEIN   Chains: A,C   EC no.: 3.1.-.-   
Polymer:
 2   Molecule: COLICIN E7   Fragment: NUCLEASE DOMAIN, RESIDUES 446-576   Mutation: YES   Chains: B,D   EC no.: 3.1.-.-   

  Authors  

Huang, H.,  Yuan, H.S.*

 

 2JB0

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CRYSTAL STRUCTURE OF THE MUTANT H573A OF THE NUCLEASE DOMAIN OF COLE7 IN COMPLEX WITH IM7

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Characteristics  

Release Date:  03-Apr-2007  Exp. Method:  X Ray Diffraction 

Resolution:  1.91 Å

  Classification  

Hydrolase/inhibitor Complex    

  Compound  

Polymer: 1   Molecule: COLICIN E7 IMMUNITY PROTEIN   Chains: A   EC no.: 3.1.-.-   
Polymer:
 2   Molecule: COLICIN E7   Fragment: NUCLEASE DOMAIN, RESIDUES 446-576   Mutation: YES   Chains: B   EC no.: 3.1.-.-   

  Authors  

Huang, H.,  Yuan, H.S.*

 

 2JBG

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CRYSTAL STRUCTURE OF THE MUTANT N560A OF THE NUCLEASE DOMAIN OF COLE7 IN COMPLEX WITH IM7

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Characteristics  

Release Date:  03-Apr-2007  Exp. Method:  X Ray Diffraction 

Resolution:  2.20 Å

  Classification  

Hydrolase/inhibitor Complex    

  Compound  

Polymer: 1   Molecule: COLICIN-E7 IMMUNITY PROTEIN   Chains: A,C   EC no.: 3.1.-.-   
Polymer:
 2   Molecule: COLICIN E7   Fragment: NUCLEASE DOMAIN, RESIDUES 446-576   Mutation: YES   Chains: B,D   EC no.: 3.1.-.-   

  Authors  

Huang, H.,  Yuan, H.S.*

 

 


Fis

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Fis

3FIS

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THE MOLECULAR STRUCTURE OF WILD-TYPE AND A MUTANT FIS PROTEIN: RELATIONSHIP BETWEEN MUTATIONAL CHANGES AND RECOMBINATIONAL ENHANCER FUNCTION OR DNA BINDING

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Characteristics  

Release Date:  31-Oct-1993  Exp. Method:  X Ray Diffraction 

Resolution:  2.30 Å

  Classification  

DNA Binding Protein    

  Compound  

Polymer: 1   Molecule: FACTOR FOR INVERSION STIMULATION (FIS)   Chains: A,B  

  Authors 

Yuan, H.S.*,  Finkel, S.E.,  Feng, J-A.,  Johnson, R.C.,  Dickerson, R.E.

 

 4FIS

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THE MOLECULAR STRUCTURE OF WILD-TYPE AND A MUTANT FIS PROTEIN: RELATIONSHIP BETWEEN MUTATIONAL CHANGES AND RECOMBINATIONAL ENHANCER FUNCTION OR DNA BINDING

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 Characteristics  

Release Date:  31-Oct-1993  Exp. Method:  X Ray Diffraction 

Resolution:  2.30 Å

  Classification  

DNA Binding Protein    

  Compound  

Polymer: 1   Molecule: FACTOR FOR INVERSION STIMULATION (FIS)   Chains: A,B  

  Authors  

Yuan, H.S.*,  Finkel, S.E.,  Feng, J.-A.,  Johnson, R.C.,  Dickerson, R.E.

 

 

 1F36

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THE CRYSTAL STRUCTURE OF FIS MUTANT K36E REVEALS THAT THE TRANSACTIVATION REGION OF THE FIS PROTEIN CONTAINS EXTENDED MOBILE BETA-HAIRPIN ARMS

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Characteristics  

Release Date:  24-Dec-1997  Exp. Method:  X Ray Diffraction 

Resolution:  2.65 Å

  Classification  

Transactivation Region    

  Compound  

Polymer: 1   Molecule: FIS   Mutation: K36E   Chains: A,B  

  Authors  

Safo, M.K.,  Yuan, H.S.*

 

1ETK

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THE CRYSTAL STRUCTURE OF E. COLI FIS MUTANT Q68A

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Characteristics  

Release Date:  11-Oct-2000  Exp. Method:  X Ray Diffraction 

Resolution:  2.10 Å

  Classification  

Transcription Activator    

  Compound  

Polymer: 1   Molecule: FACTOR FOR INVERSION STIMULATION   Mutation: Q68A   Chains: A,B  

  Authors  

Cheng, Y.S.,  Yang, W.Z.,  Johnson, R.C.,  Yuan, H.S.*

 

 1ETO

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THE CRYSTAL STRUCTURE OF E. COLI FIS MUTANT R71L

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Characteristics  

Release Date:  11-Oct-2000  Exp. Method:  X Ray Diffraction 

Resolution:  1.90 Å

  Classification  

Transcription Activator    

  Compound  

Polymer: 1   Molecule: FACTOR FOR INVERSION STIMULATION   Mutation: R71L   Chains: A,B  

  Authors  

Cheng, Y.S.,  Yang, W.Z.,  Johnson, R.C.,  Yuan, H.S.*

 

 1ETQ

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THE CRYSTAL STRUCTURE OF E. COLI FIS MUTANT R71Y

 

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 Characteristics  

Release Date:  11-Oct-2000  Exp. Method:  X Ray Diffraction 

Resolution:  2.80 Å

  Classification  

Transcription Activator    

  Compound  

Polymer: 1   Molecule: FACTOR FOR INVERSION STIMULATION   Mutation: R71Y   Chains: A,B,C,D  

  Authors  

Cheng, Y.S.,  Yang, W.Z.,  Johnson, R.C.,  Yuan, H.S.*

 

 1ETV

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THE CRYSTAL STRUCTURE OF E. COLI FIS MUTANT G72A

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 Characteristics  

Release Date:  11-Oct-2000  Exp. Method:  X Ray Diffraction 

Resolution:  2.00 Å

  Classification  

Transcription Activator    

  Compound  

Polymer: 1   Molecule: FACTOR FOR INVERSION STIMULATION   Mutation: G72A   Chains: A,B  

  Authors  

Cheng, Y.S.,  Yang, W.Z.,  Johnson, R.C.,  Yuan, H.S.*

 

 1ETW

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THE CRYSTAL STRUCTURE OF E. COLI FIS MUTANT G72D

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Characteristics  

Release Date:  11-Oct-2000  Exp. Method:  X Ray Diffraction 

Resolution:  2.00 Å

  Classification  

Transcription Activator    

  Compound  

Polymer: 1   Molecule: FACTOR FOR INVERSION STIMULATION   Mutation: G72D   Chains: A,B  

  Authors  

Cheng, Y.S.,  Yang, W.Z.,  Johnson, R.C.,  Yuan, H.S.*

 

 1ETX

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THE CRYSTAL STRUCTURE OF E. COLI FIS MUTANT Q74A

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Characteristics  

Release Date:  11-Oct-2000  Exp. Method:  X Ray Diffraction 

Resolution:  1.90 Å

  Classification  

Transcription Activator    

  Compound  

Polymer: 1   Molecule: FACTOR FOR INVERSION STIMULATION   Mutation: Q74A   Chains: A,B  

  Authors  

Cheng, Y.S.,  Yang, W.Z.,  Johnson, R.C.,  Yuan, H.S.*

 

 1ETY

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THE CRYSTAL STRUCTURE OF E. COLI WILD-TYPE FIS

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 Characteristics  

Release Date:  11-Oct-2000  Exp. Method:  X Ray Diffraction 

Resolution:  2.00 Å

  Classification  

Transcription Activator    

  Compound  

Polymer: 1   Molecule: FACTOR FOR INVERSION STIMULATION   Chains: A,B  

  Authors  

Cheng, Y.S.,  Yang, W.Z.,  Johnson, R.C.,  Yuan, H.S.*

vvn

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Vvn

 1OUO

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Crystal structure of the periplasmic endonuclease Vvn

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Characteristics  

Release Date:  05-Aug-2003  Exp. Method:  X Ray Diffraction 

Resolution:  2.30 Å

  Classification  

Hydrolase    

  Compound  


Polymer:
 1   Molecule: Nuclease   Fragment: residue 19-228   Chains: A   EC no.: 3.1.-.-   

  Authors  

Yuan, H.S.*,  Li, C.L.

 

 1OUP

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Crystal structure of the periplasmic endonuclease Vvn complexed with octamer double stranded DNA

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Characteristics  

Release Date:  05-Aug-2003  Exp. Method:  X Ray Diffraction 

Resolution:  2.30 Å

  Classification  

Hydrolase/DNA    

  Compound  


Polymer:
 1   Molecule: 5'-D(*GP*CP*GP*AP*TP*CP*GP*C)-3'   Chains: C,D,F  
Polymer:
 2   Molecule: 5'-D(*GP*CP*GP*AP*TP*C)-3'   Chains: E  
Polymer:
 3   Molecule: 5'-D(P*GP*C)-3'   Chains: G  
Polymer:
 4   Molecule: Nuclease   Fragment: residues 19-231   Mutation: H80A   Chains: A,B   EC no.: 3.1.-.-   

  Authors  

Yuan, H.S.*,  Li, C.-L.

 

 2IVK

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CRYSTAL STRUCTURE OF THE PERIPLASMIC ENDONUCLEASE Vvn COMPLEXED WITH A 16-BP DNA

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Characteristics  

Release Date:  02-Jan-2007  Exp. Method:  X Ray Diffraction 

Resolution:  2.90 Å

  Classification  

Hydrolase/DNA    

  Compound  


Polymer:
 1   Molecule: 5'-D(*GP*AP*AP*TP*TP*CP*GP*AP*TP*CP *GP*AP*AP*TP*T)-3'   Chains: E,G  
Polymer:
 2   Molecule: 5'-D(*AP*AP*TP*TP*CP*GP*AP*TP*CP*GP *AP*AP*TP*TP*C)-3'   Chains: F,H  
Polymer:
 3   Molecule: 5'-D(*GP*AP*AP*TP*TP*CP*GP*AP*TP*CP *GP*AP*AP*TP*TP*C)-3'   Chains: I,J  
Polymer:
 4   Molecule: ENDONUCLEASE I   Mutation: YES   Chains: A,B,C,D   EC no.: 3.1.-.-   

  Authors  

Wang, Y.T.,  Yang, W.J.,  Li, C.L.,  Doudeva, L.G.,  Yuan, H.S.*


enzyme 

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 Other nzymes

 1MVE

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Crystal structure of a natural circularly-permutated jellyroll protein: 1,3-1,4-beta-D-glucanase from Fibrobacter succinogenes

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  Characteristics  

Release Date:  15-Jul-2003  Exp. Method:  X Ray Diffraction 

Resolution:  1.70 Å

  Classification  

Hydrolase     

  Compound  

Polymer: 1   Molecule: Truncated 1,3-1,4-beta-D-glucanase   Fragment: Residues 1-243   Chains: A   EC no.: 3.2.1.73 Go to IUBMB EC entry   

  Authors  

Tsai, L.-C.,  Shyur, L.-F.,  Lee, S.-H.,  Lin, S.-S.,  Yuan, H.S.*

 

 

 3TAT

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TYROSINE AMINOTRANSFERASE FROM E. COLI

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  Characteristics  

Release Date:  12-Aug-1999  Exp. Method:  X Ray Diffraction 

Resolution:  3.50 Å

  Classification  

Aminotransferase     

  Compound  

Polymer: 1   Molecule: TYROSINE AMINOTRANSFERASE   Chains: A,B,C,D,E,F   EC no.: 2.6.1.57 Go to IUBMB EC entry   Other Details: CONTAINS A PYRIDOXAL 5'-PHOSPHATE (PLP) COVALENTLY BOUND TO LYS 258  

  Authors  

Ko, T.P.,  Yang, W.Z.,  Wu, S.P.,  Tsai, H.,  Yuan, H.S.*



tsn_64

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Tudor-SN

3BDL

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Crystal structure of a truncated human Tudor-SN

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  Characteristics 

Release Date:  26-Aug-2008  Exp. Method:  X Ray Diffraction 

Resolution:  1.90 Å

  Classification  

Hydrolase    

  Compound  

Polymer: 1   Molecule: Staphylococcal nuclease domain-containing protein 1   Fragment: TSN-64 (SN3, SN4, Tudor, SN5 domains)   Chains: A   EC no.: 3.1.31.1 Go to IUBMB EC entry   

  Authors  

Li, C.L



img_pnpase

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Pnpase


 3CDI Download PDB FileView PDB FileStructure Visualization with Jmol Viewer Crystal structure of E. coli PNPase
View Structure Summary
  Characteristics   Release Date:  09-Dec-2008  Exp. Method:  X Ray Diffraction 
Resolution:  2.60 Å
  Classification   Transferase    
  Compound   Polymer: 1   Molecule: Polynucleotide phosphorylase   Fragment: residues 18-734   Chains: A   EC no.: 2.7.7.8 Go to IUBMB EC entry   
  Authors   Shi, Z.,  Yang, W.Z.,  Lin-Chao, S.,  Chak, K.F.,  Yuan, H.S.
 
 3CDJ Download PDB FileView PDB FileStructure Visualization with Jmol Viewer Crystal structure of the E. coli KH/S1 domain truncated PNPase
View Structure Summary
  Characteristics   Release Date:  09-Dec-2008  Exp. Method:  X Ray Diffraction 
Resolution:  2.80 Å
  Classification   Transferase    
  Compound   Polymer: 1   Molecule: Polynucleotide phosphorylase   Fragment: C terminal S1/KH truncated PNPase   Chains: A   EC no.: 2.7.7.8 Go to IUBMB EC entry   
  Authors   Shi, Z.,  Yang, W.Z.,  Lin-Chao, S.,  Chak, K.F.,  Yuan, H.S.