IMB205

 1% Agarose gel

1g Agarose 加入100ml 1X TAE buffer 中利用微波爐加熱至完全溶解後，冷卻至 60℃左右，再到入鑄膠模內，並插上齒梳，待凝固後即可使用

50X TAE (Tris-Acetate-EDTA) Stock Solution

(2M Tris-acetate, 50mM EDTA. pH8.0 at 25°C)

For each litre of solution:

242 g Tris Base (MW=121.1)

57.1 mL Glacial Acetic Acid

100 mL 0.5 M EDTA (pH 8.0)

mix Tris with stir bar to dissolve in about 600 mL of ddH2O.

add the EDTA and Acetic Acid.

bring final volume to 1 L with ddH2O.

store at room temperature.

Final working buffer concentration (1X): 40mM Tris acetate, 1 mM EDTA.