HNH endonuclease bacterial toxin Colicin E7

Factor for  inversion stimulation Fis

Periplasmaic endonuclease Vvn

Other Enzymes

Tudor-SN

Pnpase

Tdp43

Apoptoic Nuclease

RNase T

Colicin

1CEI

STRUCTURE DETERMINATION OF THE COLICIN E7 IMMUNITY PROTEIN (IMME7) THAT BINDS SPECIFICALLY TO THE DNASE-TYPE COLICIN E7 AND INHIBITS ITS BACTERIOCIDAL ACTIVITY

Characteristics	Release Date:  12-Mar-1997  Exp. Method:  X Ray Diffraction
	Resolution:  1.80 Å
Classification	Antibacterial Protein
Compound	Polymer: 1   Molecule: COLICIN E7 IMMUNITY PROTEIN   Chains: A
Authors	Chak, K.-F.,  Safo, M.K.,  Ku, W.-Y.,  Hsieh, S.-Y.,  Yuan, H.S.*

1UNK

STRUCTURE OF COLICIN E7 IMMUNITY PROTEIN

Characteristics	Release Date:  07-Jan-1998  Exp. Method:  X Ray Diffraction
	Resolution:  1.80 Å
Classification	Immunity Protein
Compound	Polymer: 1   Molecule: COLICIN E7   Chains: A,B,C,D
Authors	Ko, T.-P.,  Hsieh, S.-Y.,  Ku, W.-Y.,  Tseng, M.-Y.,  Chak, K.-F.,  Yuan, H.S.*

7CEI

THE ENDONUCLEASE DOMAIN OF COLICIN E7 IN COMPLEX WITH ITS INHIBITOR IM7 PROTEIN

Characteristics	Release Date:  17-Sep-1999  Exp. Method:  X Ray Diffraction
	Resolution:  2.30 Å
Classification	Immune System
Compound	Polymer: 1   Molecule: PROTEIN (COLICIN E7 IMMUNITY PROTEIN)   Chains: A   Polymer: 2   Molecule: PROTEIN (COLICIN E7 IMMUNITY PROTEIN)   Fragment: ENDONUCLEASE DOMAIN   Chains: B
Authors	Ko, T.P.,  Liao, C.C.,  Ku, W.Y.,  Chak, K.F.,  Yuan, H.S.*

1M08

Crystal structure of the unbound nuclease domain of ColE7

Characteristics	Release Date:  11-Dec-2002  Exp. Method:  X Ray Diffraction
	Resolution:  2.10 Å
Classification	Hydrolase
Compound	Polymer: 1   Molecule: Colicin E7   Fragment: Nuclease Domain   Chains: A,B EC no.: 3.1.-.-
Authors	Cheng, Y.S.,  Hsia, K.C.,  Doudeva, L.G.,  Chak, K.F.,  Yuan, H.S.*

1MZ8

CRYSTAL STRUCTURES OF THE NUCLEASE DOMAIN OF COLE7/IM7 IN COMPLEX WITH A PHOSPHATE ION AND A ZINC ION

Characteristics	Release Date:  23-Dec-2002  Exp. Method:  X Ray Diffraction
	Resolution:  2.00 Å
Classification	Toxin     Hydrolase/protein Binding
Compound	Polymer: 1   Molecule: Colicin E7 immunity protein   Chains: A,C Polymer: 2   Molecule: Colicin E7   Fragment: nuclease domain   Chains: B,D   EC no.: 3.1.-.-
Authors	Sui, M.J.,  Tsai, L.C.,  Hsia, K.C.,  Doudeva, L.G.,  Ku, W.Y.,  Han, G.W.,  Yuan, H.S.*

1PT3

Crystal structures of nuclease-ColE7 complexed with octamer DNA

Characteristics	Release Date:  30-Mar-2004  Exp. Method:  X Ray Diffraction
	Resolution:  2.50 Å
Classification	Hydrolase/DNA
Compound	Polymer: 1   Molecule: 5'-GCGATCGC-3'   Chains: C,D,E,F,G,H Polymer: 2   Molecule: Colicin E7   Fragment: residues 449-576   Chains: A,B   EC no.: 3.1.-.-
Authors	Hsia, K.C.,  Chak, K.F.,  Cheng, Y.S.,  Ku, W.Y.,  Yuan, H.S.*

1ZNS

Crystal structure of N-ColE7/12-bp DNA/Zn complex

Characteristics	Release Date:  14-Mar-2006  Exp. Method:  X Ray Diffraction
	Resolution:  2.50 Å
Classification	Hydrolase/DNA
Compound	Polymer: 1   Molecule: 5'-D(*CP*GP*GP*GP*AP*TP*AP*TP*CP*CP*CP*G)-3'   Chains: B,C   Polymer: 2   Molecule: Colicin E7   Fragment: nuclease domain   Mutation: H545E   Chains: A   EC no.: 3.1.-.-
Authors	Doudeva, L.G.,  Huang, H.,  Hsia, K.C.,  Shi, Z.,  Li, C.L.,  Shen, Y.,  Yuan, H.S.*

1ZNV

How a His-metal finger endonuclease ColE7 binds and cleaves DNA with a transition metal ion cofactor

Characteristics	Release Date:  14-Mar-2006  Exp. Method:  X Ray Diffraction
	Resolution:  2.00 Å
Classification	Hydrolase/protein Binding
Compound	Polymer: 1   Molecule: Colicin E7 immunity protein   Chains: A,C Polymer: 2   Molecule: Colicin E7   Fragment: Nuclease domain   Mutation: H545E   Chains: B,D   EC no.: 3.1.-.-
Authors	Doudeva, L.G.,  Huang, H.,  Hsia, K.C.,  Shi, Z.,  Li, C.L.,  Shen, Y.,  Yuan, H.S.

2AXC

Crystal structure of ColE7 translocation domain

Characteristics	Release Date:  14-Mar-2006  Exp. Method:  X Ray Diffraction
	Resolution:  1.70 Å
Classification	Hydrolase
Compound	Polymer: 1   Molecule: Colicin E7   Fragment: N-terminal Translocation domain   Chains: A   EC no.: 3.1.-.-
Authors	Cheng, Y.S.,  Shi, Z.,  Doudeva, L.G.,  Yang, W.Z.,  Chak, K.F.,  Yuan, H.S.

2IVH

CRYSTAL STRUCTURE OF THE NUCLEASE DOMAIN OF COLE7 (H545Q MUTANT) IN COMPLEX WITH AN 18-BP DUPLEX DNA

Characteristics	Release Date:  02-Jan-2007  Exp. Method:  X Ray Diffraction
	Resolution:  2.80 Å
Classification	Hydrolase/DNA
Compound	Polymer: 1   Molecule: 5'-D(*GP*GP*AP*AP*TP*TP*CP*GP*AP*TP *CP*GP*AP*AP*TP*TP*CP*C)-3'   Chains: B,C   Polymer: 2   Molecule: COLCIN-E7   Fragment: NUCLEASE DOMAIN, RESIDUES 449-576   Mutation: YES   Chains: A   EC no.: 3.1.-.-
Authors	Wang, Y.T.,  Yang, W.J.,  Li, C.L.,  Doudeva, L.G.,  Yuan, H.S.

2JAZ

CRYSTAL STRUCTURE OF THE MUTANT N560D OF THE NUCLEASE DOMAIN OF COLE7 IN COMPLEX WITH IM7

Characteristics	Release Date:  03-Apr-2007  Exp. Method:  X Ray Diffraction
	Resolution:  2.03 Å
Classification	Hydrolase/inhibitor Complex
Compound	Polymer: 1   Molecule: COLICIN E7 IMMUNITY PROTEIN   Chains: A,C EC no.: 3.1.-.-    Polymer: 2   Molecule: COLICIN E7   Fragment: NUCLEASE DOMAIN, RESIDUES 446-576   Mutation: YES   Chains: B,D   EC no.: 3.1.-.-
Authors	Huang, H.,  Yuan, H.S.*

2JB0

CRYSTAL STRUCTURE OF THE MUTANT H573A OF THE NUCLEASE DOMAIN OF COLE7 IN COMPLEX WITH IM7

Characteristics	Release Date:  03-Apr-2007  Exp. Method:  X Ray Diffraction
	Resolution:  1.91 Å
Classification	Hydrolase/inhibitor Complex
Compound	Polymer: 1   Molecule: COLICIN E7 IMMUNITY PROTEIN   Chains: A   EC no.: 3.1.-.- Polymer: 2   Molecule: COLICIN E7   Fragment: NUCLEASE DOMAIN, RESIDUES 446-576   Mutation: YES   Chains: B   EC no.: 3.1.-.-
Authors	Huang, H.,  Yuan, H.S.*

2JBG

CRYSTAL STRUCTURE OF THE MUTANT N560A OF THE NUCLEASE DOMAIN OF COLE7 IN COMPLEX WITH IM7

Characteristics	Release Date:  03-Apr-2007  Exp. Method:  X Ray Diffraction
	Resolution:  2.20 Å
Classification	Hydrolase/inhibitor Complex
Compound	Polymer: 1   Molecule: COLICIN-E7 IMMUNITY PROTEIN   Chains: A,C EC no.: 3.1.-.-    Polymer: 2   Molecule: COLICIN E7   Fragment: NUCLEASE DOMAIN, RESIDUES 446-576   Mutation: YES   Chains: B,D   EC no.: 3.1.-.-
Authors	Huang, H.,  Yuan, H.S.*

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Fis

3FIS

THE MOLECULAR STRUCTURE OF WILD-TYPE AND A MUTANT FIS PROTEIN: RELATIONSHIP BETWEEN MUTATIONAL CHANGES AND RECOMBINATIONAL ENHANCER FUNCTION OR DNA BINDING

Characteristics	Release Date:  31-Oct-1993  Exp. Method:  X Ray Diffraction
	Resolution:  2.30 Å
Classification	DNA Binding Protein
Compound	Polymer: 1   Molecule: FACTOR FOR INVERSION STIMULATION (FIS)   Chains: A,B
Authors	Yuan, H.S.*,  Finkel, S.E.,  Feng, J-A.,  Johnson, R.C.,  Dickerson, R.E.

4FIS

THE MOLECULAR STRUCTURE OF WILD-TYPE AND A MUTANT FIS PROTEIN: RELATIONSHIP BETWEEN MUTATIONAL CHANGES AND RECOMBINATIONAL ENHANCER FUNCTION OR DNA BINDING

Characteristics	Release Date:  31-Oct-1993  Exp. Method:  X Ray Diffraction
	Resolution:  2.30 Å
Classification	DNA Binding Protein
Compound	Polymer: 1   Molecule: FACTOR FOR INVERSION STIMULATION (FIS)   Chains: A,B
Authors	Yuan, H.S.*,  Finkel, S.E.,  Feng, J.-A.,  Johnson, R.C.,  Dickerson, R.E.

1F36

THE CRYSTAL STRUCTURE OF FIS MUTANT K36E REVEALS THAT THE TRANSACTIVATION REGION OF THE FIS PROTEIN CONTAINS EXTENDED MOBILE BETA-HAIRPIN ARMS

Characteristics	Release Date:  24-Dec-1997  Exp. Method:  X Ray Diffraction
	Resolution:  2.65 Å
Classification	Transactivation Region
Compound	Polymer: 1   Molecule: FIS   Mutation: K36E   Chains: A,B
Authors	Safo, M.K.,  Yuan, H.S.*

1ETK

THE CRYSTAL STRUCTURE OF E. COLI FIS MUTANT Q68A

Characteristics	Release Date:  11-Oct-2000  Exp. Method:  X Ray Diffraction
	Resolution:  2.10 Å
Classification	Transcription Activator
Compound	Polymer: 1   Molecule: FACTOR FOR INVERSION STIMULATION   Mutation: Q68A Chains: A,B
Authors	Cheng, Y.S.,  Yang, W.Z.,  Johnson, R.C.,  Yuan, H.S.*

1ETO

THE CRYSTAL STRUCTURE OF E. COLI FIS MUTANT R71L

Characteristics	Release Date:  11-Oct-2000  Exp. Method:  X Ray Diffraction
	Resolution:  1.90 Å
Classification	Transcription Activator
Compound	Polymer: 1   Molecule: FACTOR FOR INVERSION STIMULATION   Mutation: R71L Chains: A,B
Authors	Cheng, Y.S.,  Yang, W.Z.,  Johnson, R.C.,  Yuan, H.S.*

1ETQ

THE CRYSTAL STRUCTURE OF E. COLI FIS MUTANT R71Y

Characteristics	Release Date:  11-Oct-2000  Exp. Method:  X Ray Diffraction
	Resolution:  2.80 Å
Classification	Transcription Activator
Compound	Polymer: 1   Molecule: FACTOR FOR INVERSION STIMULATION   Mutation: R71Y   Chains: A,B,C,D
Authors	Cheng, Y.S.,  Yang, W.Z.,  Johnson, R.C.,  Yuan, H.S.*

1ETV

THE CRYSTAL STRUCTURE OF E. COLI FIS MUTANT G72A

Characteristics	Release Date:  11-Oct-2000  Exp. Method:  X Ray Diffraction
	Resolution:  2.00 Å
Classification	Transcription Activator
Compound	Polymer: 1   Molecule: FACTOR FOR INVERSION STIMULATION   Mutation: G72A Chains: A,B
Authors	Cheng, Y.S.,  Yang, W.Z.,  Johnson, R.C.,  Yuan, H.S.*

1ETW

THE CRYSTAL STRUCTURE OF E. COLI FIS MUTANT G72D

Characteristics	Release Date:  11-Oct-2000  Exp. Method:  X Ray Diffraction
	Resolution:  2.00 Å
Classification	Transcription Activator
Compound	Polymer: 1   Molecule: FACTOR FOR INVERSION STIMULATION   Mutation: G72D   Chains: A,B
Authors	Cheng, Y.S.,  Yang, W.Z.,  Johnson, R.C.,  Yuan, H.S.*

1ETX

THE CRYSTAL STRUCTURE OF E. COLI FIS MUTANT Q74A

Characteristics	Release Date:  11-Oct-2000  Exp. Method:  X Ray Diffraction
	Resolution:  1.90 Å
Classification	Transcription Activator
Compound	Polymer: 1   Molecule: FACTOR FOR INVERSION STIMULATION   Mutation: Q74A Chains: A,B
Authors	Cheng, Y.S.,  Yang, W.Z.,  Johnson, R.C.,  Yuan, H.S.*

1ETY

THE CRYSTAL STRUCTURE OF E. COLI WILD-TYPE FIS

Characteristics	Release Date:  11-Oct-2000  Exp. Method:  X Ray Diffraction
	Resolution:  2.00 Å
Classification	Transcription Activator
Compound	Polymer: 1   Molecule: FACTOR FOR INVERSION STIMULATION   Chains: A,B
Authors	Cheng, Y.S.,  Yang, W.Z.,  Johnson, R.C.,  Yuan, H.S.*

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Vvn

1OUO

Crystal structure of the periplasmic endonuclease Vvn

Characteristics	Release Date:  05-Aug-2003  Exp. Method:  X Ray Diffraction
	Resolution:  2.30 Å
Classification	Hydrolase
Compound	Polymer: 1   Molecule: Nuclease   Fragment: residue 19-228   Chains: A   EC no.: 3.1.-.-
Authors	Yuan, H.S.*,  Li, C.L.

1OUP

Crystal structure of the periplasmic endonuclease Vvn complexed with octamer double stranded DNA

Characteristics	Release Date:  05-Aug-2003  Exp. Method:  X Ray Diffraction
	Resolution:  2.30 Å
Classification	Hydrolase/DNA
Compound	Polymer: 1   Molecule: 5'-D(*GP*CP*GP*AP*TP*CP*GP*C)-3'   Chains: C,D,F   Polymer: 2   Molecule: 5'-D(*GP*CP*GP*AP*TP*C)-3'   Chains: E   Polymer: 3   Molecule: 5'-D(P*GP*C)-3'   Chains: G   Polymer: 4   Molecule: Nuclease   Fragment: residues 19-231   Mutation: H80A Chains: A,B   EC no.: 3.1.-.-
Authors	Yuan, H.S.*,  Li, C.-L.

2IVK

CRYSTAL STRUCTURE OF THE PERIPLASMIC ENDONUCLEASE Vvn COMPLEXED WITH A 16-BP DNA

Characteristics	Release Date:  02-Jan-2007  Exp. Method:  X Ray Diffraction
	Resolution:  2.90 Å
Classification	Hydrolase/DNA
Compound	Polymer: 1   Molecule: 5'-D(*GP*AP*AP*TP*TP*CP*GP*AP*TP*CP *GP*AP*AP*TP*T)-3'   Chains: E,G   Polymer: 2   Molecule: 5'-D(*AP*AP*TP*TP*CP*GP*AP*TP*CP*GP *AP*AP*TP*TP*C)-3'   Chains: F,H   Polymer: 3   Molecule: 5'-D(*GP*AP*AP*TP*TP*CP*GP*AP*TP*CP *GP*AP*AP*TP*TP*C)-3'   Chains: I,J   Polymer: 4   Molecule: ENDONUCLEASE I   Mutation: YES   Chains: A,B,C,D   EC no.: 3.1.-.-
Authors	Wang, Y.T.,  Yang, W.J.,  Li, C.L.,  Doudeva, L.G.,  Yuan, H.S.*

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Other nzymes

1MVE

Crystal structure of a natural circularly-permutated jellyroll protein: 1,3-1,4-beta-D-glucanase from Fibrobacter succinogenes

Characteristics	Release Date:  15-Jul-2003  Exp. Method:  X Ray Diffraction
	Resolution:  1.70 Å
Classification	Hydrolase
Compound	Polymer: 1   Molecule: Truncated 1,3-1,4-beta-D-glucanase   Fragment: Residues 1-243   Chains: A   EC no.: 3.2.1.73
Authors	Tsai, L.-C.,  Shyur, L.-F.,  Lee, S.-H.,  Lin, S.-S.,  Yuan, H.S.*

3TAT

TYROSINE AMINOTRANSFERASE FROM E. COLI

Characteristics	Release Date:  12-Aug-1999  Exp. Method:  X Ray Diffraction
	Resolution:  3.50 Å
Classification	Aminotransferase
Compound	Polymer: 1   Molecule: TYROSINE AMINOTRANSFERASE   Chains: A,B,C,D,E,F   EC no.: 2.6.1.57  Other Details: CONTAINS A PYRIDOXAL 5'-PHOSPHATE (PLP) COVALENTLY BOUND TO LYS 258
Authors	Ko, T.P.,  Yang, W.Z.,  Wu, S.P.,  Tsai, H.,  Yuan, H.S.*

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Tudor-SN

Crystal structure of a truncated human Tudor-SN

Characteristics	Release Date:  26-Aug-2008  Exp. Method:  X Ray Diffraction
	Resolution:  1.90 Å
Classification	Hydrolase
Compound	Polymer: 1 Molecule: Staphylococcal nuclease domain-containing protein 1 Fragment: TSN-64 (SN3, SN4, Tudor, SN5 domains) Chains: A EC no.: 3.1.31.1
Authors	Li, C.L

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Pnpase

3CDI

Crystal structure of E. coli PNPase

Characteristics	Release Date:  09-Dec-2008  Exp. Method:  X Ray Diffraction
	Resolution:  2.60 Å
Classification	Transferase
Compound	Polymer: 1   Molecule: Polynucleotide phosphorylase   Fragment: residues 18-734   Chains: A   EC no.: 2.7.7.8
Authors	Shi, Z.,  Yang, W.Z.,  Lin-Chao, S.,  Chak, K.F.,  Yuan, H.S.

3CDJ

Crystal structure of the E. coli KH/S1 domain truncated PNPase

Characteristics	Release Date:  09-Dec-2008  Exp. Method:  X Ray Diffraction
	Resolution:  2.80 Å
Classification	Transferase
Compound	Polymer: 1   Molecule: Polynucleotide phosphorylase   Fragment: C terminal S1/KH truncated PNPase   Chains: A   EC no.: 2.7.7.8
Authors	Shi, Z.,  Yang, W.Z.,  Lin-Chao, S.,  Chak, K.F.,  Yuan, H.S.

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TDP43

3D2W	Crystal structure of mouse TDP-43 RRM2 domain in complex with DNA

 Characteristics  

Release Date:  07-Apr-2009  Exp. Method:  X Ray Diffraction 

Resolution:  1.65 Å

  Classification  

DNA/RNA Binding Protein    

  Compound  

Polymer: 1
Molecule: TAR DNA-binding protein 43	Chains: A	type: polypeptide(L)	length: 89
Fragment: RRM2 motif, UNP residues 192-265
Polymer: 2
Molecule: DNA (5'-D(*DGP*DTP*DTP*DGP*DAP*DGP*DCP*DGP*DTP*DT)-3')	Chains: B	type: polydeoxyribonucleotide	length: 40

  Authors  

Kuo, P.H.,  Yuan, H.S

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Apoptotic Nueleases

3CG7	Crystal structure of cell-death related nuclease 4 (CRN-4)

  Characteristics  

Release Date:  30-Dec-2008  Exp. Method:  X Ray Diffraction 

Resolution:  2.50 Å

  Classification  

Hydrolase     Apoptosis    

  Compound  

Polymer: 1
Molecule: Cell death-related nuclease 4	Chains: A, B	type: polypeptide(L)	length: 308	EC no.: 3.1.-.-

  Authors  

Hsiao, Y.-Y.,  Yuan, H.S.

3Cm5	Crystal structure of cell-death related nuclease 4 (CRN-4) bound with Mn

  Characteristics  

Release Date:  30-Dec-2008  Exp. Method:  X Ray Diffraction 

Resolution:  2.81 Å

  Classification  

Hydrolase     Apoptosis    

  Compound  

Polymer: 1
Molecule: Cell death-related nuclease 4	Chains: A, B	type: polypeptide(L)	length: 308	EC no.: 3.1.-.-

  Authors  

Hsiao, Y.-Y.,  Yuan, H.S.

3CM6	Crystal structure of cell-death related nuclease 4 (CRN-4) bound with Er

  Characteristics  

Release Date:  30-Dec-2008  Exp. Method:  X Ray Diffraction 

Resolution:  2.60 Å

  Classification  

Hydrolase     Apoptosis    

  Compound  

Polymer: 1
Molecule: Cell death-related nuclease 4	Chains: A, B	type: polypeptide(L)	length: 308	EC no.: 3.1.-.-

  Authors  

Hsiao, Y.-Y.,  Yuan, H.S.

3HKM

Crystal Structure of rice(Oryza sativa) Rrp46

Authors:

Yang, C.-C.,   Wang, Y.-T.,   Hsiao, Y.-Y.,   Doudeva, L.G.,   Yuan, H.S.

Release Date:

2010-01-26

Classification:

Hydrolase

Experiment:

X-RAY DIFFRACTION with resolution of 1.98 Å

Compound:

1 Polymer 

Molecule:	Os03g0854200 protein
Polymer:	1	Type:	polypeptide(L)	Length:	246
Chains:	A, B, C
EC#:	3.1.13.-

Citation:

Not Available.

Crystal Structure of C. elegans cell-death-related nuclease 5(CRN-5)

3KRN

Authors:

Yang, C.-C.,   Wang, Y.-T.,   Hsiao, Y.-Y.,   Doudeva, L.G.,   Chow, S.Y.,   Yuan, H.S.

Release Date:

2010-01-26

Classification:

Hydrolase

Experiment:

X-RAY DIFFRACTION with resolution of 3.92 Å

Compound:

1 Polymer 

Molecule:	Protein C14A4.5, confirmed by transcript evidence
Polymer:	1	Type:	polypeptide(L)	Length:	222
Chains:	A, B
EC#:	3.1.13.-

Citation:

Not Available.

RNase T


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3NGY

Crystal structure of RNase T (E92G mutant)

Authors:

Hsiao, Y.-Y.,   Yuan, H.S.

Release Date:

2011-02-16

Classification:

Hydrolase

Experiment:

X-RAY DIFFRACTION with resolution of 2.20 Å 

Compound:

Molecule:	Ribonuclease T
Polymer:	1	Type:	polypeptide(L)	Length:	235
Chains:	A, B, C, D
EC#:	3.1.13.-
Mutation:	E92G
Molecule:	his tag sequence
Polymer:	2	Type:	polypeptide(L)	Length:	6
Chains:	E

Image	Identifier	Name	Formula
	CO	COBALT (II) ION	Co

2 Polymers [ Display Full Polymer Details | Display for All Results ]
1 Ligand [ Display Full Ligand Details | Display for All Results ]

Citation:

PubMed Abstract:
RNA maturation relies on various exonucleases to remove nucleotides successively from the 5' or 3' end of nucleic acids. However, little is known regarding the molecular basis for substrate and cleavage preference of exonucleases. Our biochemical and structural analyses on RNase T-DNA complexes show that the RNase T dimer has an ideal architecture for binding a duplex with a short 3' overhang to produce a digestion product of a duplex with a 2-nucleotide (nt) or 1-nt 3' overhang, depending on the composition of the last base pair in the duplex. A 'C-filter' in RNase T screens out the nucleic acids with 3'-terminal cytosines for hydrolysis by inducing a disruptive conformational change at the active site. Our results reveal the general principles and the working mechanism for the final trimming step made by RNase T in the maturation of stable RNA and pave the way for the understanding of other DEDD family exonucleases.

Citation Authors:
Hsiao, Y.-Y.,   Yang, C.-C.,   Lin, C.L.,   Lin, J.L.J.,   Duh, Y.,   Yuan, H.S.

Structural basis for RNA trimming by RNase T in stable RNA 3'-end maturation
(2011) Nat.Chem.Biol. [ Display Full Abstract | Display for All Results ]

3NGZ

Crystal structure of RNase T in complex with a non-preferred ssDNA (GC) with one Mg in the active site

Authors:

Hsiao, Y.-Y.,   Yuan, H.S.

Release Date:

2011-02-16

Classification:

Hydrolase/DNA

Experiment:

X-RAY DIFFRACTION with resolution of 2.10 Å

Compound:

Molecule:	Ribonuclease T
Polymer:	1	Type:	polypeptide(L)	Length:	235
Chains:	A, B
EC#:	3.1.13.-
Mutation:	E92G
Molecule:	5'-D(P*GP*C)-3'
Polymer:	2	Type:	polydeoxyribonucleotide	Length:	2
Chains:	C, D
Other Details:	ssDNA

Image	Identifier	Name	Formula
	CO	COBALT (II) ION	Co
	MG	MAGNESIUM ION	Mg

2 Polymers [ Display Full Polymer Details | Display for All Results ]
2 Ligands [ Display Full Ligand Details | Display for All Results ]

Citation:

PubMed Abstract:
RNA maturation relies on various exonucleases to remove nucleotides successively from the 5' or 3' end of nucleic acids. However, little is known regarding the molecular basis for substrate and cleavage preference of exonucleases. Our biochemical and structural analyses on RNase T-DNA complexes show that the RNase T dimer has an ideal architecture for binding a duplex with a short 3' overhang to produce a digestion product of a duplex with a 2-nucleotide (nt) or 1-nt 3' overhang, depending on the composition of the last base pair in the duplex. A 'C-filter' in RNase T screens out the nucleic acids with 3'-terminal cytosines for hydrolysis by inducing a disruptive conformational change at the active site. Our results reveal the general principles and the working mechanism for the final trimming step made by RNase T in the maturation of stable RNA and pave the way for the understanding of other DEDD family exonucleases.

Citation Authors:
Hsiao, Y.-Y.,   Yang, C.-C.,   Lin, C.L.,   Lin, J.L.J.,   Duh, Y.,   Yuan, H.S.

Structural basis for RNA trimming by RNase T in stable RNA 3'-end maturation
(2011) Nat.Chem.Biol. [ Display Full Abstract | Display for All Results ]

3NH0

Crystal structure of RNase T in complex with a non-preferred ssDNA (AAC)

Authors:

Hsiao, Y.-Y.,   Yuan, H.S.

Release Date:

2011-02-16

Classification:

Hydrolase/DNA

Experiment:

X-RAY DIFFRACTION with resolution of 2.30 Å

Compound:

Molecule:	Ribonuclease T
Polymer:	1	Type:	polypeptide(L)	Length:	235
Chains:	A, B
EC#:	3.1.13.-
Molecule:	5'-D(*TP*TP*AP*CP*AP*AP*C)-3'
Polymer:	2	Type:	polydeoxyribonucleotide	Length:	7
Chains:	C, D
Other Details:	ssDNA

2 Polymers [ Display Full Polymer Details | Display for All Results ]

Citation:

PubMed Abstract:
RNA maturation relies on various exonucleases to remove nucleotides successively from the 5' or 3' end of nucleic acids. However, little is known regarding the molecular basis for substrate and cleavage preference of exonucleases. Our biochemical and structural analyses on RNase T-DNA complexes show that the RNase T dimer has an ideal architecture for binding a duplex with a short 3' overhang to produce a digestion product of a duplex with a 2-nucleotide (nt) or 1-nt 3' overhang, depending on the composition of the last base pair in the duplex. A 'C-filter' in RNase T screens out the nucleic acids with 3'-terminal cytosines for hydrolysis by inducing a disruptive conformational change at the active site. Our results reveal the general principles and the working mechanism for the final trimming step made by RNase T in the maturation of stable RNA and pave the way for the understanding of other DEDD family exonucleases.

Citation Authors:
Hsiao, Y.-Y.,   Yang, C.-C.,   Lin, C.L.,   Lin, J.L.J.,   Duh, Y.,   Yuan, H.S.

Structural basis for RNA trimming by RNase T in stable RNA 3'-end maturation
(2011) Nat.Chem.Biol. [ Display Full Abstract | Display for All Results ]