2020
Wu*, C.C., Lin*,
J.L.J. and Yuan*, H. S.
Structures, mechanisms and
functions of His-Me finger nucleases.
Trends Biochem. Sci. (2020).
Lin, C.-C., Chen, Y. P., Yang, W. Z., Shen, J.C.K., and Yuan*, H. S
Structural insights into CpG-specific DNA methylation by human DNA methyltransferase 3B
Nucleic Acids Res.. 48, 3949-3961 (2020).
2019Wu, C.C., Lin, J.L.J., Yang-Yen, H.-F. and Yuan*,
H. S.
A unique exonuclease ExoG cleaves at the junction
between RNA and DNA in mitochondrial DNA replication.
Nucleic Acids Res. 47, 5405-5419 (2019).
Chu,
L.-Y., Agrawal, S., Chen, Y. P., Yang,
W. Z. and Yuan*, H. S.
Structural insights into nanoRNA degradation by human Rexo2.
RNA 25, 737-746 (2019).
Agrawal, S., Kuo, P.-H., Chu, L.-Y., Golzarroshan, B., Jain, M. and Yuan*, H.
S.
RNA recognition motifs of disease-linked
RNA-binding proteins contribute to amyloid formation.
Sci. Rep. 9, 6171 (2019).
2018
Golzarroshan, B., Lin, C.-L., Li, C.-L., W.-Z. Yang, Chu, L.-Y., Agrawal, S. and Yuan*, H. S.
Crystal
structure of dimeric human PNPase reveals why disease-linked mutants
suffer from low RNA import and degradation activities.
Nucleic Acids Res. 46,
8630-8640
(2018).
Li, C.-L., W.-Z. Yang, Shi, Z. and Yuan*, H. S.
Tudor
staphylococcal nuclease is a structure-specific ribonuclease that
degrades RNA at unstructured regions during microRNA decay.
RNA 24, 739-748 (2018).
2017
Chu, L.-Y., Hsieh, T.-J., Golzarroshan, B., Chen, Y.-P., Agrawal, S. and Yuan*, H. S.
Structural insights into RNase R in RNA unwinding and degradation. Nucleic Acids Res. 45,12015–12024(2017).
Huen, J., Lin, C.-L., Yi, W.-L., W.-Z. Yang, Golzarroshan, B. and Yuan*, H. S.
Structural insights into a unique dimeric DEAD-box helicase CshA that promotes RNA decay. Structure 25, 469–481 (2017).
2016Lin, J. L. J., Wu, C.-C., Yang, W.-Z. and and Yuan*, H. S.
Crystal structure of endonuclease G in complex with DNA reveals how it nonspecifically degrades DNA as a homodimer.
Nucleic Acids Res. 44,10480-10490 (2016).
Huang, K.-W., Hsu, K.-C., Chu, L.-Y., Yang, J.-M. Yuan*, H. S. and Hsiao*, Y.-Y.
Identification
of inhibitors for the DEDDh family of exonucleases and a unique
inhibition mechanism revealed by crystal structure analysis of CRN-4
bound with 2-morpholin-4-ylethanesulfonate (MES). J. Med. Chem. 59(17):8019-29 (2016).
Lin, J. L. J., Nakagawa, A., Skeen-Gaar, R. Yang, W.-Z., X., Ge, S., Mitani, Xue*, D. and Yuan*, H. S.
Oxidative stress impairs cell death by repressing the nuclease activity of Endonuclease G. Cell Rep.12, 279-287 (2016).
Zhou,
Q., Li, H., Li, H., Nakagawa, A., Lin, J. L. J., Lee, E.-S., Harry,
B.L., Skleen-Gaar, R., Shehiro, Y., William, D., Mitani, S., Yuan, H.
S., Kang*, B.-H., Xue*, D.
Mitochondrial endonuclease G mediates breakdown of paternal mitochondria upon fertilization.
Science 353, 394-399 (2016).
Lee, Y.-M., Duh, Y., Wang, S.-T.,
Lai*, M.M.C., Yuan*, H. S. and Lim*, C.
Using an old drug to target a new drug site:
Application of disulfiram to target the Zn-site in HCV NS5A protein.
J. Am.
Chem. Soc. 138, 3856-62 (2016)
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Chiang, C. -H., Grauffel, C. Wu, L.-S., Kuo, P.-H., Doudeva, L. G, Lim,
C., Shen, C.K.J. and Yuan*, H. S.
Structural analysis of disease-related TDP-43 D169G mutation: linking
enhanced stability and caspase cleavage
efficiency to protein accumulation.
Sci. Rep. 6: 21581 (2016).
2015
Duh, Y., Hsiao, Y.-Y., Li, C.-L., Huang, J. C. and Yuan*, H. S.
Aromatic residues in RNase T stack with nucleobases to guide the sequence-specific
recognition and cleavage of nucleic acids.
Protein Sci. 24, 1934-1941 (2015).
2014
Hsiao, Y.-Y., Fang, W.-H., Lee, C.-C, Chen, Y.-P., Hsu, P.-C. and Yuan*, H. S.
Structural insights into DNA repair by RNase T, an exonuclease processing 3′ end of structured DNA in repair pathways. PLoS Biol. 12, 3, e1001803 (2014).
Kuo,
P.-H, Chiang, C.-H, Wang, Y.-T., Doudeva, L. G., and Yuan*, H. S.
The
crystal structure of TDP-43 RRM1-DNA complex reveals the specific recognition
for UG- and TG-rich nucleic acids. Nucleic Acids Res. 42, 4712-4722 (2014).
Chen, Y.-J., Li, C.-L., Hsiao, Y.-Y., Duh, Y. and Yuan*, H. S.
Structure and function of TatD exonuclease in DNA repair. Nucleic Acids Res.10776-10785 (2014).
Fang, Y.-S.,
Chang, Y.-J.,
Kuo, P.-H.,
Tsai, K.-J.,
Liao, J.Y.,
Chou, S,-C,
Lin V.,
Yuan, H. S.,
Cheng, I. H,
Tu, P.-H.,
and Chen*,Y.-R.
Full-length TDP-43 forms toxic amyloid oligomers that are present in
frontotemporal lobar dementia-TDP patients. Nature Comm., 5, 4824 (2014).
2013
Lee, Y.-M., Wang, Y.-T., Duh, Y., Yuan*, H. S. and Lim*, C.
Identification of labile Zn sites in drug-target proteins.
J. Am. Chem. Soc. 2013, 135, 4028-4031 (2013).
Wang, Y. -T., Kuo, P.
-H., Chiang, C. -H, Liang., J -R., Chen, Y. -R., Wang, S., Shen, J.C.K., and Yuan*, H. S.
The truncated C-terminal RNA recognition motif of TDP-43 protein plays a key role in forming proteinaceous aggregates.J. Biol. Chem. 2013, 288, 13, 9049–9057 (2013).
2012Su. M. W., Yuan, S. H. and W. C. Chu*
Recombination in the
non-structural gene region in type-2 dengue viruses.
Intervirol. 2012, 55,
225-230 (2012).
Hsiao, Y.-Y., Duh, Y., Chen, Y.-P., Wang, Y.-T., and Yuan*, H. S.
How an exonuclease decides where to stop in trimming of nucleic acids: crystal structures of RNase T-product complexes. Nucleic Acids Res. 40, 8144-8154 (2012).
Lin, J. L. J., Lin, C. L., Hsiao, Y. Y., Doudeva, L. G., Yang, W.-Z., Wang, Y.-T. and Yuan*, H. S.
Structural insights into DNA binding and cleavage by CED-3 Protease Suppressor-6 (CPS-6) from Caenorhabditis elegans.J. Biol. Chem. 287, 10, 7110-7120 (2012).
Lin C. L., Wang, Y.-T, Hsiao, Y.-Y., Yang, W.-Z. and Yuan*, H. S.
Crystal structure of human polynucleotide phosphorylase: Insights into its domain function in RNA binding and degradation. Nucleic Acids Res. 40, 4146-4157(2012).
2011
Hsiao, Y.-Y., Yang, C.-C, Lin C. L., Lin, J.
L. J., Duh, Y. and Yuan*, H. S. Structural basis for RNA trimming by RNase T in stable RNA 3'-end
maturation.Nature Chem.
Biol. 7, 236-243(2012).2010
Yang, C.-C, Wang, Y.-T., Hsiao, Y. Y., Doudeva, L. G., Kuo, P.-H., Chow, S. Y. and Yuan*, H. S.
Structural and biochemical characterization of CRN-5 and Rrp46: an exosome component participating in apoptotic DNA degradation.RNA
16:
1748-1759 (2010).
Fu,
C. C., Lin, C. F., Gao, Q. Z., Yang, W. Z., Lim, T. S., Yang, L. L,
Yen, C. F., Chang, W. H., Yuan, H. S., Sheu*, S. Y., Yang*, D. Y.,
Fann, W.
Fis-protein induces rod-like DNA bending.
Chem. Phys. Letter 500, 318-322 (2010).
2009Su, M.-W., Lin, H.-M., Yuan*, H. S. and Chu*, W.-CCategorizing host-dependent RNA viruses by principal component analysis of their coden usage preferences. J. Comp. Biol. 16, 1539-1547 (2009).Wang, Y.-T., Wright, J, D., Doudeva, L. G., H -C., Lim*, C. and Yuan*, H. S. Redesign of high-affinity nonspecifid nucleases with altered sequence preference.J. Am. Chem. Soc. 2009,
131,
17345-17353 (2009).Hsiao, Y.
Y., Nakagawa, A., Shi, Z., Mitani, S., Xue, D. and Yuan*, H.
S. Crystal structure of CRN-4: implications for
domain function in apoptotic DNA degradation. Mol. Cell. Biol. 29, 2, 448-457 (2009).
Lin, Y.-S., Tsai; L.-C., Lee, S.-H., Yuan,
H. S. and Shyur*, L.-F.
Structural and catalytic roles of residues
located in beta13 strand and the following beta-turn loop in Fibrobacter succinogenes
1,3-1,4- beta -D-glucanase.
Biochim. Biophys.
Acta. 1790, 231–239 (2009).
Kuo,
P,-S, Doudeva, L. G., Wang, Y.-T., Shen, C.-K.
J. and Yuan*, H. S. Structural insights into TDP-43 in nucleic
acid binding and domain interactions. Nucleic Acids Res. 37, 6, 1799-1808 (2009).
2008Shi, Z., Yang, W.-Z.,
Lin-Chao, S., Chak, K.-F. and Yuan*, H. S.
Crystal structure of Escherichia
coli PNPase: central channel residues are
involved in processive RNA degradation.RNA 14, 1-11 (2008).
Li, C. -L.,Yang, W. -Z., Chen, Y. -P. and Yuan*, H. S.
Structural and functional
insights into human Tudor-SN, a key component linking RNA interference and
editing.
Nucleic
Acid Res. 36,
11, 3579-3589 (2008).
2007
Lei, G. -S., Chen, C. -J., Yuan, H.
S., Wang., S. -H. and Hu*, S. -T.
Inhibition of IS2 transposition by factor for
inversion stimulation.
FEMS Microbiol
Lett. 275, 98-105 (2007).
Huang,
H. and Yuan*, H. S.
The conserved asparagine in the HNH motif plays an
important structural role in metal finger endonucleases.
J.
Mol. Biol. 368, 3, 812-821 (2007).
Wang, Y. -T., Yang, Y. -J., Li, C.
-L., Doudeva, L. G., and Yuan*, H. S.
Structural basis for sequence-dependent DNA cleavage by
nonspecific endonucleases.
Nucleic
Acid Res. 35, 2, 584-594
(2007).
2006
Pan, Y. -H., Liao, C. -C., Kuo, C. -C., Duan, K.-J., Liang,
P.-H, Yuan, H. S., Hu, S.-T. and Chak*, K.-F.
The critical
roles of polyamines in regulating ColE7 production and restricting ColE7 uptake
of the colicin-producing Escherichia coli.
J. Biol. Chem. 281, 13083 (2006).
Doudeva, L. G.,
Huang, H., Hsia, K.-C., Shi, Z., Li, C. -L., Cheng, Y. -S. and
Yuan*, H. S.
Crystal
structural analysis and metal-dependent stability and activity studies of the
ColE7 endonuclease domain in complex with DNA/Zn2+ or inhibitor/Ni2+.
Protein Sci. 15, 269-280 (2006).
Cheng,
Y.-S., Shi, Z., Doudeva, L. G., Yang, W. -Z., Chak, K. -F. and Yuan*, H. S.
High-resolution crystal structure of a truncated ColE7
translocation domain: Implications for colicin transport across membranes.
J.
Mol. Biol. 356, 22-31 (2006).
2005
Hsia, K.-C., Li, C. -L. and
Yuan*, H. S.
Structural and functional insight into the sugar-nonspecific
nucleases in host defense.
Curr. Opin. Struct. Biol. 15, 126-134 (2005).Liu,
H., Peng, H.-W., Cheng, Y.-S., Yuan, H. S.
and Yang-Yen*, H.-F.
Stabilization and enhancement of the
antiapoptotic activity of Mcl-1 by TCTP.
Mol.
Cell. Biol. 3117–3126 (2005).
Shi, Z.,
Chak. K.-F. and Yuan*, H. S.
Identification of an essential cleavage site in
ColE7 required for import and killing of cells.
J.
Biol. Chem.
2005, 280, 24663-24668
(2005).
2004
Hsia, K.-C., Chak,
K.-F., Liang, P. -H., Cheng, Y. -S., Ku, W. -Y. and Yuan*, H.
S.
DNA binding and degradation by
the H-N-H protein ColE7
Structure 12, 205-214 (2004).
Lin, Y.-H., Liao, C. -C., Liang, P. -H., Yuan*, H. S. and Chak*, K. -F.
Involvement of colicin in the
limited protection of the colicin producing cells against bacteriophage
Biochem. Biophys. Res. Commun. 318, 81-87 (2004).
2003
Tsai, L. -C., Shyur,
L. -F., Lee, S. -H., Lin, S. -S. and Yuan*, H.
S.
Crystal structure of a natural
circularly-permutated jellyroll protein: 1,3-1,4-beta-D-glucanase
from Fibrobacter succinogenes.
J. Mol. Biol. 330, 607-620 (2003).
Li, C. -L., Hor,
L. -I., Chang, Z. -F., Tsai, L. -C., Yang, W. -Z. and Yuan*, H.
S.
DNA binding and
cleavage by the periplasmic nuclease Vvn- A novel structure with a known active
site.
EMBO J. 22, 4014-4025 (2003).
2002
Chang, S. -J., Hsieh, S. -Y., Yuan, H. S.
and Chak*, K.-F.
Characterization
of the specific cleavage of ceiE7-mRNA of the bactericidal ColE7 operon.
Biochem. Biophys. Res. Commun. 299, 613-620 (2002).
Cheng, Y. -S., Hsia, K. -C., Doudeva, L. G., Kin-Fu Chak and
Yuan*, H. S.
The crystal structure of the nuclease
domain of colicin E7 suggests a mechanism for binding to double-stranded DNA by
the H-N-H endonucleases.
J. Mol. Biol. 324, 227-236 (2002).
Sui, M. -J., Tsai, L. -C., Hsia, K. -C., Doudeva, L. G., Ku, W.-Y., Han, G. W. and Yuan*, H. S.
Metal ions and phosphate
binding in the H-N-H motif: Crystal structures of the nuclease domain of
ColE7/Im7 in complex with a phosphate ion and different divalent metal ions.
Protein Sci. 11, 2947-2957 (2002).
Cheng, H. -L., Tsai, L. -C., Lin, S. -S.,
Yuan*, H. S., Yang, N. -S., Lee, S. -H. and Shyur*, L. -F.
Mutagenesis of Trp54 and
Trp203 residues on Fibrobacter Succinogens 1,3-1,4-b-D-glucanase
significantly affects catalytic activities of the enzyme.
Biochemistry 2002, 41, 8759-8766
(2002).
Ku, W. -Y., Liu, Y. -W., Hsu, Y. -C., Liao,
C. -C., Liang, P. -H., Yuan, H. S. and Chak*, K. -F.
The zinc ion in the HNH motif of the
endonuclease domain of colicin E7 is not required for DNA binding but is
essential for DNA hydrolysis.
Nucleic Acid Res. 30, 1670-1678 (2002).
Yang Z., Zhang H., Hung, H. -C., Kuo,
C. -C., Tsai, L. -C., Yuan, H. S., Chou, W. -Y., Chang, G. -G., Tong*, L.
Structural studies of the
pigeon cytosolic NADP(+)-dependent malic enzyme.
Protein Sci. 11:332-41 (2002).
2001
Chen, J. L., Tsai, L. C., Wen, T. N., Tang, J. B.,
Yuan*, H. S. and Shyur*, L. F.
Directed mutagenesis of specific active site residues on
Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase
significantly affects catalysis and enzyme structural stability.
J. Biol. Chem. 276, 17895-17901 (2001).
Tsai, L. C., Shyur, L. F., Lin, S.
S., Yuan*, H. S.
Crystallization
and preliminary X-ray diffraction analysis of the 1,3-1,4-beta-D-glucanase
from Fibrobacter succinogenes.
Acta Cryst. D. 57, 1303-1306 (2001).
2000
Cheng,
Y.-S., Yang, W.-Z., Johnson, R. C. and Yuan*, H. S.
Structure analysis of the
transcriptional activation region on Fis: Crystal structures of Six Fis Mutants
with Different activation properties.
J.
Mol. Biol. 302, 1139-1151 (2000).
1999
Ko, T.-P., Liao, C.-C., Ku,
W.-Y., Chak, K.-F. and
Yuan*, H. S.
The crystal
structure of the DNase domain of colicin E7 in complex with its inhibitor Im7
protein.
Structure 7, 91-102 (1999).
Tsai, L.-C, Kuo,
C.-C., Chou, W.-Y., Chang, G.-G. and Yuan*, H.
S.
Crystallization
and preliminary x-ray diffraction analysis of malic enzyme from pigeon liver.
Acta Cryst. D. 55, 1930-1932 (1999).
Ko, T.-P., Yang, W.-Z, Wu,
S.-P., Hsin, T., and Yuan*, H. S.
The preliminary crystallographic analysis of the
open-form E. coli tyrosine aminotransfer ase
bound with a cofactor pyridoxal 5'-phosphate.
Acta Cryst. D. 55, 1474-1477 (1999).
Lu, F.-M., Yuan*, H. S., Hsu, Y.-C.,
Chang, S.-J., Chak*, K.-F.
Hierarchical
order of critical residues on the immunity-determining region of the Im7
protein which confer specific immunity to its cognate colicin.
Biochem. Biophys. Res. Commun. 264, 69-75 (1999).
Chang*, S.-L., Chao, C.-H., Huang, Y.-S.,
Jean, Y.-C., Sheu, H.-S., Liang, F.-J., Chen, C.-K.,
Yuan*, H. S.
Quantitative phase determination for macromolecular crystals
using stereoscopic multibeam imaging
Acta. Cryst. A. 55, 933-938 (1999).
Hsu, W.-H., Chien,
F.-T., Hsu, C.-L., Wang, T.-C., Yuan*, H. S., and Wang*, W.-C.
Expression, crystallization
and preliminary x-ray diffraction studies of N- carbamyl-D-amino-acid
amidohydrolase from Agrobacterium radiobacter.
Acta. Cryst. D. 55, 694-695 (1999).
1998
Yang, W.-Z, Ko,
T. P., Corselli, L., Johnson, R. C. and Yuan*, H. S.
The conversion from a
beta-strand to an alph-helix induced by a single-site mutation identified in
the crystal structures of Fis mutant Pro26Ala.
Protein Sci. 7, 1875-1883 (1998).
1997
Safo, M. K.,
Yang, W.-Z., Corselli, L., Crampton,
S., Yuan*, H. S, and Johnson*, R. C.
The transactivation region
of the Fis protein that controls site-specific DNA inversion contains extended
mobile beta-hairpin arms.
EMBO J. 16, 6860-6873 (1997).
Hsieh,
S. -H., Ko, T. -P., Tseng,
M. -Y., Ku, W. -Y., Chak*, K.-F, and Yuan*, H. S.
A novel role of ImmE7 in the
autoregulatory expression of ColE7 operon and identification of possible
ribonuclease active sites in the crystal structure of dimeric ImmE7.
EMBO
J. 16,
1444-1454 (1997).
1996
Chak, K.-F., Safo, M. K., Ku, W.-Y., Hsieh, S.-Y., and Yuan*, H. S.
The crystal structure of the ImmE7 protein
suggests a possible colicin-interacting surface.
Proc.
Natl. Acad. Sci. USA 93, 6437-6442 (1996).
1995
Ku,
W. -Y., Wang, C. -S., Chen, J. -Y., Chak, K.-F., Safo, M. K. and Yuan*, H. S.
Crystallization
and preliminary crystallographic analysis of ImmE7 protein of Colicin E7.
Proteins, Struc. Func. &
Genet. 23, 588-590 (1995).
1994
Yuan*,
H. S., Yang, W.-Z., Wang, S. S., Finkel, S. E. and
Johnson, R. C.
The structure of Fis
mutant Pro61-Ala illustrates that the kink within the long a-helix is not due
to the presence of the proline residue.
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Biol. Chem. 269, 46, 28947-28954 (1994). Yuan*,
H. S., Stevens, R. C., Kotzle, T. F. and Bau*, R.
Determination
of the absolute configuration of (S)-(+)-neopentyl-1-d alcohol by neutron
diffraction study of strychnium neopentylphthalate.
Proc.
Natl. Acad. Sci. USA 91, 12872-12876 (1994).